The discoveries from our laboratory link molecular structure and mechanism to cell physiology in both health and disease. Our descriptions of complex intracellular processes at remarkable spatial and temporal resolutions have set new standards for understanding membrane dynamics in cells, by bridging from atomic detail to cell morphology through implementation of frontier imaging technologies, such as lattice light-sheet microscopy.  With these type of dynamic studies we integrate molecular snapshots obtained at high resolution with live-cell processes, in an effort to generate other ‘molecular movies’ allowing us to obtain new frameworks for analyzing some of the molecular contacts and switches that participate in the regulation, availability, and intracellular traffic of the many molecules involved in signal transduction, immune responsiveness, lipid homeostasis, cell-cell recognition and organelle biogenesis. Such biological phenomena have importance for our understanding of many diseases including cancer, viral infection and pathogen invasion, Alzheimer’s, as well as other neurological diseases